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Welcome to the CaliAli Documentation

CaliAli is a comprehensive suite designed for extracting neural signals from one-photon calcium imaging data collected across multiple sessions in free-moving conditions. CaliAli incorporates all the necessary modules to extract long-term tracked calcium signals from raw video sessions.

CaliAli Pipeline

Key Features

The CaliAli pipeline follows the following steps

  1. Downsampling and conversion of video to CaliAli format.
  2. Motion Correction.
  3. Automatic Detrending and Preprocessing.
  4. Alignment of Sessions.
  5. Neural Signal Extraction from concatenated videos with a computationally efficient approach.
  6. Post-processing and Evaluation

Getting Started